He'd been battling intestinal issues for months and had surgery in November, forcing The Eagles to pull out of appearances.
Glenn was one of the founding members of the band. He co-wrote and sang most of the Eagles hits, including Hotel California, Take It Easy, Tequila Sunrise, Lyin' Eyes and Heartache Tonight.
The star was a major part of The Eagles, helping them land in the Rock And Roll Hall Of Fame with several classics.
When the band split up in 1980, Frey went off on his own and found success. His single The Heat Is On was a chart topper after it was in the film Beverly Hills Cop (1984). He also came out with You Belong to the City, which was used often in commercials. During his solo career, Frey had 12 charting songs in the US Top 100, and he also appeared in several episodes of Miami Vice.
In 1994 he reunited with The Eagles. They launched their Hell Freezes Over tour, which was a massive success.
As a member of The Eagles, Frey won six Grammy Awards, and five American Music Awards. The Eagles have sold over 120 million albums worldwide and were inducted into the Rock and Roll Hall of Fame in 1998, the first year they were nominated.
He is survived by his wife Cindy with whom he has three children. Glenn Frey was 67.